Physical Biochemistry serves as a comprehensive guide to the principles and techniques essential for the study of biological macromolecules. Over the years, it has been refined through extensive use in academic courses and valuable feedback from students and reviewers. This book reflects the latest advancements in the field, integrating numerous new techniques that have become vital in recent years. Key topics covered include helix-coil transitions, cot analysis, buffer systems, metal-ion electrodes, high-speed autoradiography, and new immunoelectrophoresis methods. It also discusses Southern, Northern, and Western transfers, light scattering, ligand binding, DNA sequencing, and fluctuation spectroscopy. Techniques such as agarose-gel chromatography of DNA, once briefly mentioned, are now extensively covered due to their widespread use in DNA research. The book has been meticulously updated with input from experts and incorporates the latest developments from laboratory supply companies, particularly Bio-Rad Laboratories. Expanded sections on Raman and NMR spectroscopy, affinity and hydroxyapatite chromatography, macromolecule structure, viscoelasticity, electron microscopy, scintillation counting, and gas chromatography ensure comprehensive coverage of the field. Physical Biochemistry remains an invaluable resource for students and professionals, providing clear explanations and up-to-date methodologies that are crucial for modern biochemistry and molecular biology research.
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